Opendata, web and dolomites

RecPAIR SIGNED

Genetic landscape of the homology search

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 RecPAIR project word cloud

Explore the words cloud of the RecPAIR project. It provides you a very rough idea of what is the project "RecPAIR" about.

movements    defects    integrity    breaks    sequencing    genetic    central    coli    microscopy    gene    screen    faithful    strains    mutagenesis    homology    perhaps    phenotyping    microfluidic    throughput    genes    double    drives    broken    genome    probability    linked    template    pooled    ageing    limit    vulnerable    hr    incorrect    recombination    strands    uses    interrupted    position    molecular    library    first    functional    induction    single    homologous    dsb    phenotypic    replicated    directed    screening    quality    characterised    combination    fluorescent    repair    cancer    map    internal    duplex    threatening    sources    damage    situ    crispr    dcas9    cell    refined    tested    phenotype    recover    crispri    chromosome    center    lies    landscape    dumpling    genotyping    dynamics    create    elusive    lesions    pairs    coupled    search    external    strand    monitored    dsbs    model    vivo    life    localises    dna    characterisation    conclude    characterise   

Project "RecPAIR" data sheet

The following table provides information about the project.

Coordinator
UPPSALA UNIVERSITET 

Organization address
address: VON KRAEMERS ALLE 4
city: UPPSALA
postcode: 751 05
website: www.uu.se

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Sweden [SE]
 Total cost 191˙852 €
 EC max contribution 191˙852 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2019
 Duration (year-month-day) from 2019-06-08   to  2021-06-07

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UPPSALA UNIVERSITET SE (UPPSALA) coordinator 191˙852.00

Map

 Project objective

The integrity of genetic information is central to life, yet the DNA is vulnerable to damage from internal and external sources. Incorrect repair of DNA damage drives mutagenesis, loss of genetic information, ageing, and cancer. Double strand DNA breaks (DSBs) are perhaps the most threatening DNA lesions, where the integrity of both strands of the DNA duplex is interrupted at the same position. In E. coli, faithful repair of DSBs is possible only through the homologous recombination (HR) pathway which uses replicated chromosome as a template to recover the information. At the center of HR lies an elusive search process, during which broken strand localises and pairs with the repair template.

I will use a combination of CRISPR/dCas9 screening and in-situ genotyping of pooled library of strains to characterise the genetic landscape controlling the homology search. First, I will develop a low probability DSB induction method, to limit the DSB-formation to only a single chromosome per cell. Next, I will design and implement a whole-genome CRISPRi screen coupled to high-throughput sequencing and map the genes involved specifically in the homology directed repair of DSBs. The knowledge of the recombination-specific genes will allow to create a refined, high-quality phenotypic screen. In this screen the whole chromosome dynamics will be monitored and defects in the DNA movements will be characterised for each tested target with a microfluidic-based fluorescent microscopy. Each phenotype will be linked to a specific gene using the state-of-the-art in-situ phenotyping approach called DuMPLING. The functional characterisation of recombination genes will allow to conclude a molecular model of the search process in vivo.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "RECPAIR" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "RECPAIR" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

TheaTheor (2018)

Theorizing the Production of 'Comedia Nueva': The Process of Play Configuration in Spanish Golden Age Theater

Read More  

CHES (2020)

Resilience of Coastal Human-Environment Systems

Read More  

MarshFlux (2020)

The effect of future global climate and land-use change on greenhouse gas fluxes and microbial processes in salt marshes

Read More