PROTEOMEXCHANGE

International Data Exchange and Data Representation Standards for Proteomics

 Partecipanti

Mappa

 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

 Obiettivo del progetto (Objective)

'Over the last few years the field of proteomics has evolved into a prolific data producer. As a result, various databases that collect and redistribute the acquired data have been established. While data format standards for quantitative proteomics have now been defined and implemented with significant contribution from the recently completed EU ProDaC grant, standards for quantitative proteomics are still lacking. This simultaneous creation of multiple repositories and databases, and lack of standards for quantitative proteomics result in a fragmentation of data, and cause confusion for data submitters and users alike. Based on consortium expertise in the operation of large scale proteomics repositories (PRIDE, PeptideAtlas, Tranche, Peptidome) we aim to implement the next step, regular data exchange between major international proteomics resources. In parallel, we will further develop standards (mzQuantML) for the dynamic field of quantitative mass spectrometry. The main objectives of ProteomExchange are user-oriented: (i) to provide a single point of data submission to the user; (ii) to ensure data availability in all of the different member databases; (iii) to use community standard formats to represent the data, so it becomes accessible to all regardless of data origin; (iv) to provide added value through different views on the same data, from repositories to derived search tools. With an international consortium and support from large scale data producers (ISAS (Germany), U. Cambridge (UK), Karolinska Institute (Sweden)), industry (Pfizer, Philips, Waters), and journals (Nature Biotechnology, MCP, JPR), we here propose a Coordination Action project to solidify an emerging informal collaboration between major repositories into a production-quality data deposition and dissemination consortium on par with the systems so successfully employed by three-dimensional structure databases and nucleotide sequence databases, amongst others.'

Introduzione (Teaser)

An EU-funded initiative has provided a common framework and the infrastructure needed for the cooperation of mass spectroscopy-based proteomics resources.

Descrizione progetto (Article)

Before the project started in 2011, among the existing proteomics repositories, the Proteomics Identifications http://www.ebi.ac.uk/pride (PRIDE) database and http://www.peptideatlas.org (Peptide Atlas) had the broadest target audience. On the one hand, PRIDE stores data as initially analysed by researchers. On the other, data in the Peptide Atlas are reprocessed with a focus on low protein false discovery rates.

The EU-funded project 'International data exchange and data representation standards for proteomics' (http://www.proteomexchange.org/ (PROTEOMEXCHANGE)) was initiated to coordinate data deposition and dissemination to such major repositories. The project consortium originally formed in 2006 with the aim to develop infrastructure enabling researchers to submit data in a consistent, harmonised format as well as to access data that were already public.

With financial support from the Seventh Framework Programme (FP7), PROTEOMEXCHANGE partners established a standard framework for proteomics data submission and dissemination to PRIDE, Peptide Atlas and other repositories. It also delivers different 'views' of the deposited data, including the raw data and the processed results, all linked by a universally shared identifier.

Individual resources can join PROTEOMEXCHANGE by following the membership agreement and implementing the data submission and metadata requirements. Authors can cite the assigned accession number for data sets used in their publications. In addition, a Digital Object Identifier (DOI) is provided for the data sets containing the results in open standard formats. This way, data sets become publishable per se and can be tracked when reported in the scientific literature.

Since its introduction in 2012, the number of submissions and downloads through PROTEOMEXCHANGE steadily increased. The upturn is indicative of an ongoing shift in mentality in the field. In the last few years, data sharing is slowly becoming common among proteomics researchers. As the repositories have improved, researchers are keener to submit their data.

In 2014, approximately four data set submissions were received daily. The total number of data sets submitted exceeded 1 600, and the volume of downloads reached 150 terabytes. By providing the means of accessing data across the individual repositories, the PROTEOMEXCHANGE framework is expected to maximise their benefit to the scientific community.