EOS

Enzyme catalysis in organic solvents

Coordinatore	CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE    more Spiacenti, non ci sono informazioni su questo coordinatore. Contattare Fabio per maggiori infomrazioni, grazie.
Nazionalità Coordinatore	France [FR]
Totale costo	1˙390˙800 €
EC contributo	1˙390˙800 €
Programma	FP7-IDEAS-ERC Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	ERC-2011-StG_20101014
Funding Scheme	ERC-SG
Anno di inizio	2012
Periodo (anno-mese-giorno)	2012-01-01   -   2016-12-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE  Organization address address: Rue Michel -Ange 3 city: PARIS postcode: 75794 contact info Titolo: Dr. Nome: Damien Cognome: Laage Email: send email Telefono: +33 1 44322418	FR (PARIS)	hostInstitution	1˙390˙800.00
2	CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE  Organization address address: Rue Michel -Ange 3 city: PARIS postcode: 75794 contact info Titolo: Ms. Nome: Julie Cognome: Zittel Email: send email Telefono: +33 1 42 34 94 16 Fax: +33 1 42 34 95 08	FR (PARIS)	hostInstitution	1˙390˙800.00

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 Obiettivo del progetto (Objective)

'Enzymes are remarkably efficient catalysts and their recent use in non-aqueous organic solvents is opening a tremendous range of applications in synthetic chemistry: since, surprisingly, most enzymes do not denature in these non-natural environments, new reactions involving e.g. water-insoluble reagents can be catalyzed, while unwanted degradation side reactions are suppressed. However, a key challenge for these applications is to overcome the greatly reduced catalytic activity compared to aqueous conditions. Empirically, adding activators such as salts or small amounts of water dramatically enhances the activity, but the underlying mechanisms have remained elusive, thus preventing a rational optimization. Through analytic modeling and numerical simulations, our project will provide the first atomic-scale detailed description of enzyme catalysis in organic solvents, including the key role of the environment. We will then use this unprecedented molecular insight to design rigorous new procedures for the rational engineering of systems with dramatically enhanced activities, both through optimized choices of solvents and additives, and through targeted protein mutations. Specifically, we will first rigorously establish the influence of enzyme flexibility on catalytic activity through an original model accounting for the dynamic disorder arising from conformation fluctuations. Second, we will provide the first molecular explanation of the commonly invoked “lubricating” action of added water. Third, the underlying mechanism of the much employed salt-induced activation will be determined, probably calling for a radical change from the currently used picture of a water-mediated action. Far-reaching practical impacts are expected for the numerous industrial syntheses already employing biocatalysis in non-aqueous media.'