MORPHOBEADS

Studying Morphogenesis by building reaction-diffusion networks in gel bead arrays

 Coordinatore STICHTING KATHOLIEKE UNIVERSITEIT 

 Organization address address: GEERT GROOTEPLEIN NOORD 9
city: NIJMEGEN
postcode: 6525 EZ

contact info
Titolo: Mr.
Nome: Niels
Cognome: Eijkemans
Email: send email
Telefono: 31243652709

 Nazionalità Coordinatore Netherlands [NL]
 Totale costo 183˙805 €
 EC contributo 183˙805 €
 Programma FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call FP7-PEOPLE-2011-IEF
 Funding Scheme MC-IEF
 Anno di inizio 2012
 Periodo (anno-mese-giorno) 2012-05-01   -   2014-04-30

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    STICHTING KATHOLIEKE UNIVERSITEIT

 Organization address address: GEERT GROOTEPLEIN NOORD 9
city: NIJMEGEN
postcode: 6525 EZ

contact info
Titolo: Mr.
Nome: Niels
Cognome: Eijkemans
Email: send email
Telefono: 31243652709

NL (NIJMEGEN) coordinator 183˙805.80

Mappa

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 Word cloud

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inhibitor    enzyme    diffusion    morphogenesis    enzymatic    reactions    cell    feedback    chemical    beads    negative    reaction    positive    self    equilibrium    gel    networks   

 Obiettivo del progetto (Objective)

'Living matter is characterized as operating far out of equilibrium, and most of the cellular non-equilibrium processes are organized in extremely complex networks. A visible demonstration of some networks is morphogenesis, which is the driving force for the formation of patterns in tissue. In this project I aim to systematically study the underlying physical and chemical components of morphogenesis in an artificial system. The main characteristics are coupling of chemical reaction and diffusion times, spatial inhomogeneities and positive or negative feedback loops. To achieve this goal, I will assemble an array of prototype ‘cells’ consisting of monodisperse microscopic gel beads produced using microfluidic devices. Within these gel beads, enzymatic reactions will take place to mimic intracellular signaling processes, while the product of the enzymatic reactions can diffuse from one bead to another, thereby resembling cell-cell communication. The enzymatic reactions chosen for my project are self-catalyzed proteolysis and kinase self-phosphorylation, both of which will generate positive feedback. A negative feedback loop will be introduced by using the peptidase cascade by hydrolysis of a pre-inhibitor, releasing a free inhibitor after cleavage. An analysis of the reaction diffusion profile is a novel method for measuring enzyme kinetics. As a spin-off from this project, I am interested to use a reaction-diffusion between two drops for the study of interaction of enzyme with inhibitors, in order to miniaturize and accelerate an inhibitor screening.'

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