LICHENOMICS

"LICHen population gENetics and genOMICS - Gene expression, neutral and adaptive genetic variation in natural populations of Peltigera membranacea"

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 Obiettivo del progetto (Objective)

'A major question regarding climate change is whether species can respond to rapid climate change and avoid extinction. Here, we address this question by studying neutral and adaptive genetic variation in populations of the lichen-forming fungus Peltigera membranacea. Based on existing genome data (host lab), we identified hundreds of microsatellites (SSR) and 119 genes involved in fungal environmental stress responsea (ESR). Landscape-level patterns of gene movement will be studied with SSR markers, offering valuable insight on whether the species is likely to spread to sites with suitable conditions under climate change (landscape genetics). An experiment will shed light on whether gene expression differs when thalli are grown at high and low temperatures (experimental transcriptomics). The ESR genes will be investigated with Next Generation Sequencing to identify SNPs with an association to environmental variables to infer the species’ potential to adapt to the environment based on standing genetic variation within populations (landscape genomics). The results will clarify the response of lichen fungi to climate change, both in terms of the species’ potential for adaptation, tolerance of changed conditions, and spread of individuals to new sites. By performing this fascinating research, the fellow will have the valuable opportunity to receive high quality training in bioinformatics including analysis of transcriptomes, genome annotation, and training in laboratory skills, i.e. isolation of high-quality mRNA and preparation of libraries for Solexa sequencing. A long-term collaboration will be established between the laboratories in Switzerland and Iceland, as a major step in building a pan-European researcher team working on population genomics of lichens (LICHENOMICS). This project will thus contribute to European excellence by strengthening a unique discipline involving numerous fascinating biological phenomena yet to be explored.'

Introduzione (Teaser)

One of the most important questions posed by climate change is whether species can respond to a changing climate quickly enough to avoid extinction. In order to survive, populations must either be able to adapt to or tolerate the change in environment, or migrate to more suitable conditions.

Descrizione progetto (Article)

Lichens are not single organisms, but instead are comprised of algae or cyanobacteria (or both) living together in partnership with a fungus. They play an important role in ecosystems through nutrient cycling, food supply and as a habitat for microorganisms. However, to date, little is known about the effects of climate change on lichen-forming fungi and their photobiont partners.

Some lichen fungi show evidence of restricted gene flow or low dispersal ability. If they are unable to tolerate conditions created by climate change, they will be at greater risk of extinction due to their inability to shift their ranges. Therefore, it is important to study gene flow patterns in lichen fungi and their potential for adaptation to a changing environment.

The LICHENOMICS project studied the ground-dwelling lichen Peltigera membranacea, which plays an important role in carbon and nitrogen cycling in ecosystems at northern latitudes. The aim was to determine whether P. membranacea can migrate to more favourable locations and whether it can cope with altered conditions in situ through changes in gene expression. Researchers also evaluated whether P. membranacea can adapt to changed climate conditions.

Using microsatellite markers, genetic differences were investigated among P. membranacea populations in two study areas of Iceland. Results showed that the fungal populations were widely different, implying low migration between sites and a limited capacity for surviving environmental change by long-distance colonisation of new habitats.

Individuals of P. membranacea with different genetic backgrounds were subjected to temperature stress in the laboratory. Gene expression levels were then quantified for environmental stress response (ESR) genes. The results showed that gene expression differed significantly between high and low temperatures in several ESR genes. This result suggested that the lichen has some ability to tolerate environmental stress in situ.

LICHENOMICS provided a clearer picture of how lichen fungi respond to climate change with regard to their ability to adapt to changed conditions and spread to new sites. The results generated in this project will enable scientists to learn more about the impacts of climate change on species and ecosystems.