MINT ENZYMES

Microbial Expression Platform for Membrane Integrated Enzymes

Coordinatore	DANMARKS TEKNISKE UNIVERSITET    more  Organization address address: Anker Engelundsvej 1, Building 101A city: KONGENS LYNGBY postcode: 2800 contact info Titolo: Ms. Nome: Susanne Cognome: Plougheld Winther Email: send email Telefono: 4545258003
Nazionalità Coordinatore	Denmark [DK]
Totale costo	100˙000 €
EC contributo	100˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2011-CIG
Funding Scheme	MC-CIG
Anno di inizio	2012
Periodo (anno-mese-giorno)	2012-07-01   -   2016-06-30

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	DANMARKS TEKNISKE UNIVERSITET  Organization address address: Anker Engelundsvej 1, Building 101A city: KONGENS LYNGBY postcode: 2800 contact info Titolo: Ms. Nome: Susanne Cognome: Plougheld Winther Email: send email Telefono: 4545258003	DK (KONGENS LYNGBY)	coordinator	100˙000.00

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 Obiettivo del progetto (Objective)

'We wish to explore how and why the architecture of the DNA code for membrane proteins vary between kingdoms and affect protein production under different growth conditions - with an unprecedented systematic approach. At the core of biotechnology is heterologous expression of proteins, i.e. to transfer genes into foreign hosts like microorganisms for protein production. One of the key challenges in protein production relates to the degeneracy of the genetic code. Genetic information flows from DNA via RNA into proteins, but the code is degenerate because different DNA/RNA-nucleotide sequences can be translated into the same protein sequence. In the DNA and RNA code, three nucleotides (a codon) are translated into one amino acid residue, and amino acids can be encoded by up to six different “synonymous” codons. Substantial evidence, from studies of soluble proteins, points to a role for this “deeper layer” of the genetic code in the proper timing of co-translational protein folding. For example, regions rich in rare codons may slow down translation to allow time for folding of specific secondary structures. However, since codon usage is not conserved like the universal genetic code, protein-folding information hidden in the DNA code is difficult to transfer from one organism to another. As a result, heterologous expression often leads to misfolded, non-functional proteins. Very limited experimental evidence exist on the connection between codon usage and production of functionally active proteins and in the case of membrane-integrated protein, no such systematic studies have been performed. The knowledge gained will be exploited to engineer new scaffolds for microbial factories. Successful designs will be a novel, invaluable tool in molecular biology and may spawn future solutions for converting from a fossil fuel-based to a bio-based industrial society'