EPIPRIMING
Keeping ready for battle: understanding fundamental mechanisms of establishment and maintenance of epigenetic marks underlying abiotic stress memory in plants
| Coordinatore | UNIVERSITY OF GLASGOW
Organization address
address: University Avenue contact info |
| Nazionalità Coordinatore | United Kingdom [UK] |
| Totale costo | 231˙283 € |
| EC contributo | 231˙283 € |
| Programma | FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) |
| Code Call | FP7-PEOPLE-2013-IEF |
| Funding Scheme | MC-IEF |
| Anno di inizio | 2014 |
| Periodo (anno-mese-giorno) | 2014-08-01 - 2016-07-31 |
Partecipanti
| # | ||||
|---|---|---|---|---|
| 1 |
UNIVERSITY OF GLASGOW
Organization address
address: University Avenue contact info |
UK (GLASGOW) | coordinator | 231˙283.20 |
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Obiettivo del progetto (Objective)
'Drought and salt stress threaten crop production worldwide. Evidence from the field that pre-exposure of plants to transient mild stress (priming) enhances their tolerance to subsequent harsher stress indicates that plants maintain a ‘memory’ of environmental events that allows them to respond more efficiently to subsequent stress. However, systematic studies on the molecular processes underlying abiotic stress priming are scarce. The proposed project takes advantage of an effective salt-priming protocol for the model plant Arabidopsis thaliana developed by the host group, and builds on recently published evidence from the host that epigenetic marks consisting of a decrease of tri-methylated lysine 27 in histone 3 (H3K27me3) underlie somatic stress memory of the primed plants. The work will focus on elucidating the molecular mechanisms for the establishment, maintenance and fading of the epigenetic memory and the role it plays for improved stress tolerance. The main objectives are the following: (1) testing the role of REF6 (a plant histone demethylase), SWI3B (a component of a chromatin-remodeling complex) and nucleosome repositioning in priming-induced H3K27me3 loss, (2) investigating the mechanisms of H3K27me3 spreading after priming, (3) assessing the effect of repeated salt exposure on H3K27me3, and (4) detailed analysis of cell-type specific H3K27me3 changes in the HKT1 gene, encoding a sodium transporter with a crucial role in plant salt tolerance. The research will use a range of modern technologies including chromatin immunoprecipitation (ChIP), next-generation (Illumina) sequencing, quantitative PCR, nucleosome mapping and the use of INTACT technology for cell-type specific ChIP, combined with refined data analysis to obtain results at a high spatio-temporal/molecular resolution. Advanced training in cutting-edge technologies, collaborations with other laboratories and significant project outcomes will greatly contribute to develop the candidate’s career.'