BT ALPHA MANNOSIDASE

Biological rational for the mannosidase super family in Bacteroides thetaiotaomicron

Coordinatore	INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSE INSAT    more  Organization address address: AVENUE DE RANGUEIL 135 city: TOULOUSE postcode: 31077 contact info Titolo: Dr. Nome: Michael J. Cognome: O'donohue Email: send email Telefono: -61559460 Fax: -61559372
Nazionalità Coordinatore	France [FR]
Totale costo	45˙000 €
EC contributo	45˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2007-2-2-ERG
Funding Scheme	MC-ERG
Anno di inizio	2008
Periodo (anno-mese-giorno)	2008-01-01   -   2010-12-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSE INSAT  Organization address address: AVENUE DE RANGUEIL 135 city: TOULOUSE postcode: 31077 contact info Titolo: Dr. Nome: Michael J. Cognome: O'donohue Email: send email Telefono: -61559460 Fax: -61559372	FR (TOULOUSE)	coordinator	0.00

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 Obiettivo del progetto (Objective)

'The human intestine is colonized by over 10 trillions symbiotic bacteria which play a critical role in human health such as preventing inflammation and cancer. These microorganisms hydrolyse complex carbohydrates polymers from our diet that are not degraded by “our” own enzymes and thus, in addition to preventing cancer and inflammation, increase the nutrients available to the human host. Indeed, recent studies have shown that the composition of the microorganisms in the large intestines influences fat deposition in the mammalian host demonstrating how these bacteria can alter metabolism in their host. The symbiotic bacterium Bacteroides thetaiotaomiron is the most abundant microoganism in the large intestine. B. thetaiotaomicron, colonises the gut by accessing both human and dietary complex carbohydrates. It synthesises over 200 glycoside hydrolases which is greater than any other bacterium studied to date indicating that the microorganism exploits complex carbohydrates as an important nutrient source. Intriguingly there has been a significant expansion in glycoside hydrolase families in which the enzymes attack polymers containing the sugar mannose which is present in complex carbohydrates display on the surface of human cells. These carbohydrate structures contain three mannose molecules and are elongated with different combination of other sugars making these complex molecules highly variable. B. thetaiotaomicron produces 23 enzymes in the glycoside hydrolase family GH92 which attacks glycosidic bonds that are linked to mannose sugars and thus they are likely to target the carbohydrates on the surface of human cells. The biological rationale for such a large number of GH92 enzymes may reflect diversity of sugars attached to mannose in human complex carbohydrates. The central objective of this project is to dissect the specificity and the mechanism of carbohydrate recognition of representatives of GH92 B. thetaiotaomicron enzymes.'