PBS

Dissecting the new “platform-binding site” on the bacterial ribosome. Role of particular ribosomal proteins in regulating translation initiation in bacteria

Coordinatore	CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE    more  Organization address address: Rue Michel -Ange 3 city: PARIS postcode: 75794 contact info Titolo: Ms. Nome: Liliane Cognome: Flabbée Email: send email Telefono: +33 1 42 34 94 17 Fax: +33 1 42 34 95 08
Nazionalità Coordinatore	France [FR]
Totale costo	0 €
EC contributo	172˙267 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-IIF-2008
Funding Scheme	MC-IIF
Anno di inizio	2010
Periodo (anno-mese-giorno)	2010-02-01   -   2012-01-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE  Organization address address: Rue Michel -Ange 3 city: PARIS postcode: 75794 contact info Titolo: Ms. Nome: Liliane Cognome: Flabbée Email: send email Telefono: +33 1 42 34 94 17 Fax: +33 1 42 34 95 08	FR (PARIS)	coordinator	172˙267.70

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 Obiettivo del progetto (Objective)

'Recent X-ray crystallographic and cryo-electron microscopy data indicate that structured mRNAs bind to the bacterial ribosome at an early step of translation initiation, to a newly characterised ribosomal site located at the “platform” of the small ribosomal subunit (the “platform-binding” site or PBS). In a second step, the mRNAs are supposed to unfold and to migrate to the P-site to interact with the initiator tRNA. The proposal aims at dissecting the role of ribosomal proteins situated at the PBS and potentially contacting the structured 5’-terminal parts of mRNAs. It is proposed to isolate mutants in the genes of the corresponding proteins that influence the in vivo translation of the lacZ gene fused to different model 5’-UTRs known to be involved with translation regulation. Once such mutations are found, global analysis of their effect will be performed and their effect will be analyzed in vitro.'