SUSY
Protein SUlfenylation in Medicago truncatula – Sinorhizobium meliloti SYmbiosis
| Coordinatore | INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE
Organization address
address: Rue De L'Universite 147 contact info |
| Nazionalità Coordinatore | France [FR] |
| Totale costo | 30˙000 € |
| EC contributo | 30˙000 € |
| Programma | FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) |
| Code Call | FP7-PEOPLE-2009-RG |
| Funding Scheme | MC-ERG |
| Anno di inizio | 2009 |
| Periodo (anno-mese-giorno) | 2009-10-31 - 2011-10-30 |
Partecipanti
| # | ||||
|---|---|---|---|---|
| 1 |
INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE
Organization address
address: Rue De L'Universite 147 contact info |
FR (PARIS CEDEX 07) | coordinator | 30˙000.00 |
Mappa
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Obiettivo del progetto (Objective)
'It now appears that plants purposefully generate Reactive Oxygen Species (ROS) such as Hydrogen Peroxide (H2O2) as signalling molecules to control various processes. There is now compelling evidence that H2O2 is an important signal in plants; this has been particularly reported in the case of plant – pathogen interactions. Symbiosis between Legumes and Rhizobia is characterized by the formation of a new organ on the root able to fix atmospheric nitrogen. More recently, the involvement of ROS during the symbiotic interaction has also been highlighted where H2O2 production must contribute to the control of the establishment and/or functioning of the symbiosis, by exerting a positive effect on both partners. On the other hand, H2O2 is known to interact directly with protein thiols, leading to the formation of a sulfenic acid group (sulfenylation) which has been suggested as a widespread mechanism by which H2O2 might affect protein function. Thus, the goal of the project is to identify the model plant Medicago truncatula H2O2 targets proteins during its interaction with Sinorhizobium meliloti. The three objectives are: i) isolate specifically the sulfenylated proteins using an affinity purification followed by their proteomic identification, ii) map the H2O2 modified cysteins and validate their role in protein fonction and iii) characterise the role of the sulfenylation during the symbiotic interaction through a functional in planta analysis. The project aims at characterizing a completely new role for H2O2 as a signalling molecule in plants. The research programme will lead to the identification and the characterisation of H2O2-driven post-translational signalling. This will lead to important breakthroughs not only in the symbiotic field but also in compatible plant pathogen interaction and more generally of plant development. In this respect, it is expected to open new fields of investigation.'