BACTERIAL INVASION
Visualizing the Early Events of Shigella Invasion at the Molecular and Cellular Level
| Coordinatore | INSTITUT PASTEUR
Organization address
address: RUE DU DOCTEUR ROUX 25-28 contact info |
| Nazionalità Coordinatore | France [FR] |
| Totale costo | 166˙145 € |
| EC contributo | 166˙145 € |
| Programma | FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) |
| Code Call | FP7-PEOPLE-2009-IEF |
| Funding Scheme | MC-IEF |
| Anno di inizio | 2010 |
| Periodo (anno-mese-giorno) | 2010-06-01 - 2012-05-31 |
Partecipanti
| # | ||||
|---|---|---|---|---|
| 1 |
INSTITUT PASTEUR
Organization address
address: RUE DU DOCTEUR ROUX 25-28 contact info |
FR (PARIS CEDEX 15) | coordinator | 166˙145.60 |
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Obiettivo del progetto (Objective)
'A key step of Shigella infection is the invasion of epithelial cells of the human colon. The contact with host cells triggers the injection of bacterial effector proteins into the host cells via the type III secretion system (T3SS). The effectors induce massive cytoskeletal remodelling (actin foci). This drives localized membrane ruffling and lamellipodial extensions that envelop and internalize the bacteria. We will investigate at molecular resolution and in three dimensions (3D) how the successive steps of the invasion process are spatially and temporally coordinated. Specifically, we will address where and how the secreting T3SS needles interact with the host cell membrane, how they trigger a spatially- organized signaling response within the host cells, and how the underlying cytoskeleton re-organizes in proximity to the entering bacterium. This will be achieved via correlative light electron microscopy (CLEM) and correlative light electron tomography (CLET). Altogether, our data will illuminate Shigella invasion process at the molecular level and in 3D. Apart from addressing important biological questions, this project will also provide me with the “know-how” of innovative technological tools to study individual events that will be extremely valuable for my success as an independent researcher in the field of host-pathogen interactions.'