SMINSULATOR

Unveiling the Roles of Chromatin Insulators in Higher-order Chromatin Architecture and Transcription Regulation one molecule at a time

 Coordinatore INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM) 

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 Nazionalità Coordinatore France [FR]
 Totale costo 1˙500˙000 €
 EC contributo 1˙500˙000 €
 Programma FP7-IDEAS-ERC
Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call ERC-2010-StG_20091118
 Funding Scheme ERC-SG
 Anno di inizio 2010
 Periodo (anno-mese-giorno) 2010-11-01   -   2015-10-31

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM)

 Organization address address: 101 Rue de Tolbiac
city: PARIS
postcode: 75654

contact info
Titolo: Dr.
Nome: Marcelo
Cognome: Nollmann Martinez
Email: send email
Telefono: +33 4 67 41 79 12
Fax: +33 4 67 41 79 13

FR (PARIS) hostInstitution 1˙500˙000.00
2    INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM)

 Organization address address: 101 Rue de Tolbiac
city: PARIS
postcode: 75654

contact info
Titolo: Mr.
Nome: William
Cognome: Lepetit
Email: send email
Telefono: +33 4 67 63 70 23
Fax: +33 4 67 63 02 92

FR (PARIS) hostInstitution 1˙500˙000.00

Mappa

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 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

regulate    chromatin    impact    structure    insulators    transcription    nucleosomes    insulator    cycle    form    molecule    dynamics    dna    proteins    regulatory    replication    cell    super    single    levels    organization    bodies   

 Obiettivo del progetto (Objective)

'Eukaryotic chromosomes are condensed into several hierarchical levels of complexity: DNA is wrapped around core histones to form nucleosomes, nucleosomes form a higher-order structure called chromatin, and chromatin is subsequently organized by long-range contacts. The conformation of chromatin at these three levels greatly influences DNA transcription. One class of chromatin regulatory proteins called insulator factors set up boundaries between heterochromatin and euchromatin and generate long-range loops. In Drosophila, three types of insulators (Su(Hw), dCTCF and BEAF) have been shown to regulate transcription and organize chromatin at the higher level by the formation of long-range interactions that were proposed to be mediated by the coalescence of several insulator proteins into clusters (insulator bodies). Our research aims to unravel the mechanism by which insulator bodies dynamically regulate chromatin structure and transcription by using single-molecule biophysics and quantitative modeling. On one hand, we will apply novel super-resolution fluorescence microscopy methods to investigate the structure and assembly dynamics of insulator bodies in single cells throughout the cell cycle and the role of their regulatory partners. On the other hand, we will reconstitute the looping activity of insulators in vitro and apply single-molecule manipulation methods to gain detailed insights into the molecular mechanisms involved in defining and regulating chromatin organization by insulators. This project has the potential to impact our understanding of several fundamental cellular processes: transcription regulation, cell-cycle dynamics, higher-order chromatin organization, and cell differentiation. The methods developed here will be directly applicable to the investigation of other nuclear super-structures, such as transcription and replication factories and Polycomb bodies, and thus will impact other research areas, such as DNA replication, transcription and cell division.'

Altri progetti dello stesso programma (FP7-IDEAS-ERC)

HYPOXICMICRORNAS (2010)

Deciphering the microRNA response to hypoxia

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INTERCOM (2010)

"The Influence of Interfaces, Confinement and Compartmentalization on Chemical Reactions"

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CHROMATINSYS (2014)

Systematic Approach to Dissect the Interplay between Chromatin and Transcription

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