SEM_SEM

SEcreted Membrane vesicles: role in the therapeutic plasticity of neural StEM cells

Coordinatore	THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE    more Spiacenti, non ci sono informazioni su questo coordinatore. Contattare Fabio per maggiori infomrazioni, grazie.
Nazionalità Coordinatore	United Kingdom [UK]
Totale costo	1˙500˙000 €
EC contributo	1˙500˙000 €
Programma	FP7-IDEAS-ERC Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	ERC-2010-StG_20091118
Funding Scheme	ERC-SG
Anno di inizio	2010
Periodo (anno-mese-giorno)	2010-12-01   -   2015-11-30

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	EUROPEAN MOLECULAR BIOLOGY LABORATORY  Organization address address: Meyerhofstrasse 1 city: HEIDELBERG postcode: 69117 contact info Titolo: Mr. Nome: Ratcliff Cognome: Tom Email: send email Telefono: +44223 492 528	DE (HEIDELBERG)	beneficiary	89˙067.60
2	THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE  Organization address address: The Old Schools, Trinity Lane city: CAMBRIDGE postcode: CB2 1TN contact info Titolo: Dr. Nome: Stefano Cognome: Pluchino Email: send email Telefono: 390226000000 Fax: 390226000000	UK (CAMBRIDGE)	hostInstitution	1˙410˙932.40
3	THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE  Organization address address: The Old Schools, Trinity Lane city: CAMBRIDGE postcode: CB2 1TN contact info Titolo: Ms. Nome: Renata Cognome: Schaeffer Email: send email Telefono: +44 1223 333543 Fax: +44 1223 332988	UK (CAMBRIDGE)	hostInstitution	1˙410˙932.40

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 Obiettivo del progetto (Objective)

'Compelling evidence exists that somatic stem cell-based therapies protect the central nervous system from chronic inflammation-driven degeneration, such as that occurring in experimental autoimmune encephalomyelitis and stroke. It was first assumed that stem cells directly replace lost/damaged cells, but it has now become clear that they are able to protect the damaged nervous system through mechanisms other than cell replacement. In immune-mediated experimental demyelination and stroke we have shown that transplanted NPCs possess a constitutive and inducible ability to mediate efficient bystander myelin repair and axonal rescue. Yet, a comprehensive understanding of the mechanisms by which NPCs exert their therapeutic impact is lacking. We envisage that major NPC functions would result from highly sophisticated horizontal communication and we attribute a key role to the transfer of secreted membrane vesicles (MVs) from NPCs to neighbouring cells. Here we will focus on defining whether this form of communication exists for NPCs, and on elucidating its molecular signature and therapeutic relevance. We will investigate the MV small RNAome using next generation deep-sequencing, computational analysis and bioinformatics tools; and demonstrate that ncRNAs from NPCs are able to affect gene expression in neighbouring cells. Functional readouts of candidate ncRNAs will be provided by semi-quantitative RT-PCR and cutting-edge molecular and cellular biology tools [eg., miRNA-regulated LV vectors; identification of ncRNA targets by quantitative proteomics] on recipient cells. Then, we will demonstrate the biological impact of the transfer of individual small ncRNAs in vitro and in vivo in rodents with experimental neurological diseases. The true innovation of this project relies in its unique peculiarity to look into an innate mechanism with the visionary focus of translating the knowledge of basal stem cell functions into innovative high clinical impact therapeutics.'