GPCR-LGIC COUPLING

Interactions between G-protein Coupled Receptors and Ligand Gated Ion Channels

Coordinatore	MIDDLE EAST TECHNICAL UNIVERSITY    more  Organization address address: DUMLUPINAR BULVARI 1 city: ANKARA postcode: 6800 contact info Titolo: Dr. Nome: ?rem Cognome: Dikmen Toker Email: send email Telefono: 903122000000 Fax: 903122000000
Nazionalità Coordinatore	Turkey [TR]
Totale costo	100˙000 €
EC contributo	100˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2010-RG
Funding Scheme	MC-IRG
Anno di inizio	2010
Periodo (anno-mese-giorno)	2010-10-01   -   2014-09-30

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	MIDDLE EAST TECHNICAL UNIVERSITY  Organization address address: DUMLUPINAR BULVARI 1 city: ANKARA postcode: 6800 contact info Titolo: Dr. Nome: ?rem Cognome: Dikmen Toker Email: send email Telefono: 903122000000 Fax: 903122000000	TR (ANKARA)	coordinator	100˙000.00

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 Obiettivo del progetto (Objective)

'Dopamine receptors are members of G-protein coupled receptor superfamily. These receptors are the key point of dopaminergic system, which controls the regulation of memory, attention, food intake, endocrine regulation, psychomotor activity and positive reinforcement. To regulate so many critically important neurological events, dopamine receptors have complex interactions with other receptors and ion channels. In this study, a trimeric complex comprising D2 receptor -which is a subtype of dopamine receptors- , A2A adenosine receptor being another G-protein coupled receptor and a ligand gated ion channel N-methyl-D-aspartate (NMDA) receptor will be investigated. The aim of this project will be the application of a novel technique we refer as SplitGFP-FRET for the first time. For this study, “Fluorescence Resonance Energy Transfer” and “Split GFP” methods will be combined so as to reveal the trimeric D2 dopamine receptor- A2A adenosine receptor-NMDA receptor interactions. With the proposed study, a fluorescence technique to analyze live cell culture will be developed. This model will potentially lead to understanding of molecular mechanisms of many neuropathological conditions like schizophrenia and Parkinson’s disease because these disorders have already been shown to be associated with dysregulations in dopaminergic systems. Therefore, this project aims to reveal detailed links between schizophrenia and interactions of D2 dopamine receptor with other receptors and ion channels. The developed live cell model will also be useful for testing anti-psychotic drugs whether they have effects on disruption of protein-protein interactions, which will, in turn, ease screening of newly designed drugs.'

Introduzione (Teaser)

A new imaging approach developed by EU-funded researchers should help provide the much-awaited molecular evidence behind neurological conditions.