NRHACC

Nicotinic acetylcholine receptors in human adrenal chromaffin cells

Coordinatore	UNIVERSIDAD AUTONOMA DE MADRID    more  Organization address address: CALLE EINSTEIN, CIUDAD UNIV CANTOBLANCO RECTORADO 3 city: MADRID postcode: 28049 contact info Titolo: Mrs. Nome: Mª Carmen Cognome: Puerta Email: send email Telefono: 34914978663 Fax: 34914975269
Nazionalità Coordinatore	Spain [ES]
Totale costo	166˙336 €
EC contributo	166˙336 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2012-IIF
Funding Scheme	MC-IIF
Anno di inizio	2013
Periodo (anno-mese-giorno)	2013-07-29   -   2015-07-28

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	UNIVERSIDAD AUTONOMA DE MADRID  Organization address address: CALLE EINSTEIN, CIUDAD UNIV CANTOBLANCO RECTORADO 3 city: MADRID postcode: 28049 contact info Titolo: Mrs. Nome: Mª Carmen Cognome: Puerta Email: send email Telefono: 34914978663 Fax: 34914975269	ES (MADRID)	coordinator	166˙336.20

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 Obiettivo del progetto (Objective)

Nicotinic acetylcholine receptors (nAChRs) containing the alpha6 subunit have been implicated in neurological and psychiatric conditions including Parkinson’s and nicotinic dependence. Despite their potential therapeutic importance, little is known about the biophysical properties of these receptors in part because of their restricted tissue distribution and poor expression in heterologous systems. Nevertheless, a recent report by Dr. Albillos’ laboratory demonstrated that the alpha6beta4 subtype is the main nAChR expressed in human adrenal chromaffin cells. This seminal report constitutes the only functional demonstration of native human alpha6beta4 nAChRs and represents a timely opportunity to gain valuable insights into its biophysical properties and to further our understanding of this receptors involvement in the stimulus-secretion response of chromaffin cells. This proposal intends to characterize the biophysical properties of native human alpha6beta4 nAChRs, their interaction with mitochondria via calcium, and their functional regulation by reactive oxygen species (ROS) produced by mitochondria under pathological conditions. The specific aims of this proposal are: 1) to characterize the alpha6-containing nAChR subtype(s) present in human adrenal chromaffin cells and to investigate the regulation of their expression by nicotine; 2) to study the functional interaction between alpha6beta4 nAChRs and mitochondria; 3) the regulation of this nAChR subtype by mitochondrial ROS.