MITOTRAFFICBYMIRO

Differential role of atypical Rho GTPases Miro-1 and Miro-2 for controlling mitochondrial dynamics and transport

Coordinatore	TATA INSTITUTE OF FUNDAMENTAL RESEARCH*TIFR    more  Organization address address: HOMI BHABHA ROAD COLABA city: 400005 postcode: 400005 contact info Titolo: Dr. Nome: Sandhya Cognome: Koushika Email: send email Telefono: +91 22 22782384 Fax: +91 22 22804611
Nazionalità Coordinatore	India [IN]
Totale costo	15˙000 €
EC contributo	15˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2013-IIF
Funding Scheme	MC-IIFR
Anno di inizio	0
Periodo (anno-mese-giorno)	0000-00-00   -   0000-00-00

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	TATA INSTITUTE OF FUNDAMENTAL RESEARCH*TIFR  Organization address address: HOMI BHABHA ROAD COLABA city: 400005 postcode: 400005 contact info Titolo: Dr. Nome: Sandhya Cognome: Koushika Email: send email Telefono: +91 22 22782384 Fax: +91 22 22804611	IN (400005)	coordinator	15˙000.00

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 Obiettivo del progetto (Objective)

'Regulated transport of mitochondria is essential for providing ATP spatio-temporaly to power nerve cell function and calcium buffering during release of neurotransmitters or post-synaptic receptor activation. The major player of this transport machinery is Mitochondrial Rho GTPases Miro-1 and Miro-2 present at the mitochondrial outer membrane which controls binding to the Kinesin for transport along microtubules. Miro-1 and 2 are paralogs shares ~60% homology in mammals (while nematode Caenorhabditis elegans and plants have 3 paralogs) while others have only one copy of Miro proteins. Using high resolution 3D live cell imaging, co-immunoprecipitation followed by mass spectrometric identification, expression of genetically encoded sensors in combination with pharmacological, electrical and optogenetic stimulation of neuronal activity in vivo, the molecular mechanism that allow two Miro proteins to tailor the spatial distribution and morphology of mitochondria will be explored. A key goal will be to explore the differential role of the Miro-1 and Miro-2 which can regulate diverse cellular processes such as constitutive and activity-dependent mitochondrial transport, ER-Mitochondria contact and calcium signalling in cultured cells and dendritic shafts and synapses in vivo. I will also try to identify novel proteins that interact with Miro-1 and Miro-2 proteins and their role in maintaining mitochondrial morphology and transport. To extend this knowledge further the role of Miro proteins in axonal transport and calcium signalling in vivo will be investigated with a deletion model in C. elegans. These studies will significantly advance our current understanding of the role played by mitochondrial trafficking for controlling diverse cellular function and may also help understand of how disrupted mitochondrial trafficking and function contribute to diseases.'