QBOL
Development of a new diagnostic tool using DNA barcoding to identify quarantine organisms in support of plant health
| Coordinatore | STICHTING DIENST LANDBOUWKUNDIG ONDERZOEK
Organization address
address: Costerweg 50 contact info |
| Nazionalità Coordinatore | Netherlands [NL] |
| Totale costo | 4˙142˙713 € |
| EC contributo | 2˙991˙433 € |
| Programma | FP7-KBBE
Specific Programme "Cooperation": Food, Agriculture and Biotechnology |
| Code Call | FP7-KBBE-2008-2B |
| Funding Scheme | CP-FP |
| Anno di inizio | 2009 |
| Periodo (anno-mese-giorno) | 2009-03-21 - 2012-09-20 |
Partecipanti
| # | ||||
|---|---|---|---|---|
| 1 |
STICHTING DIENST LANDBOUWKUNDIG ONDERZOEK
Organization address
address: Costerweg 50 contact info |
NL (WAGENINGEN) | coordinator | 564˙916.00 |
| 2 |
THE SECRETARY OF STATE FOR ENVIRONMENT, FOOD AND RURAL AFFAIRS
Organization address
address: Area 1A, Nobel House, Smith Square 17 contact info |
UK (LONDON) | participant | 390˙000.00 |
| 3 |
EIDGENOESSISCHES DEPARTEMENT FUER WIRTSCHAFT, BILDUNG UND FORSCHUNG
Organization address
address: Bundeshaus Ost contact info |
CH (BERN) | participant | 345˙664.00 |
| 4 |
INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE
Organization address
address: Rue De L'Universite 147 contact info |
FR (PARIS CEDEX 07) | participant | 340˙060.00 |
| 5 |
KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPEN - KNAW
Organization address
address: KLOVENIERSBURGWAL 29 HET TRIPPENHUIS contact info |
NL (AMSTERDAM) | participant | 305˙000.00 |
| 6 |
UNIVERSITEIT GENT
Organization address
address: SINT PIETERSNIEUWSTRAAT 25 contact info |
BE (GENT) | participant | 210˙800.00 |
| 7 |
Vlaams Gewest
Organization address
address: KONING ALBERT II LAAN 20 contact info |
BE (BRUSSEL) | participant | 210˙000.00 |
| 8 |
AARHUS UNIVERSITET
Organization address
address: Nordre Ringgade 1 contact info |
DK (AARHUS C) | participant | 170˙000.00 |
| 9 |
ALMA MATER STUDIORUM-UNIVERSITA DI BOLOGNA
Organization address
address: Via Zamboni 33 contact info |
IT (BOLOGNA) | participant | 169˙960.00 |
| 10 |
CENTRO INTERNACIONAL DE LA PAPA
Organization address
address: AVENIDA LA MOLINA 1895 contact info |
PE (Lima) | participant | 110˙753.00 |
| 11 |
NEDERLANDSE VOEDSEL EN WARENAUTORITEIT
Organization address
address: CATHARIJNESINGEL contact info |
NL (UTRECHT) | participant | 65˙075.00 |
| 12 |
AGENCE NATIONALE DE SECURITE SANITAIRE DE L'ALIMENTATION, DE L'ENVIRONNEMENT ET DU TRAVAIL
Organization address
address: 14 RUE PIERRE ET MARIE CURIE contact info |
FR (Maisons Alfort) | participant | 15˙000.00 |
| 13 |
NACIONALNI INSTITUT ZA BIOLOGIJO
Organization address
address: VECNA POT 111 contact info |
SI (LJUBLJANA) | participant | 14˙934.00 |
| 14 |
Chinese Academy of Inspection and Quarantine
Organization address
address: Chaoyang District 241 contact info |
CN (Beijing) | participant | 14˙400.00 |
| 15 |
INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA
Organization address
address: Carretera de la Coruna Km7.5 contact info |
ES (MADRID) | participant | 14˙400.00 |
| 16 |
UNIVERSITA DEGLI STUDI DI MODENA E REGGIO EMILIA
Organization address
address: VIA UNIVERSITA 4 contact info |
IT (MODENA) | participant | 13˙800.00 |
| 17 |
EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA
Organization address
address: Parque Estacao Biologica - PqEB s/n contact info |
BR (BRASILIA) | participant | 12˙825.00 |
| 18 |
Nome Ente NON disponibile
Organization address
address: State phytosanitary administration 23 contact info |
CZ (Olomouc) | participant | 12˙206.00 |
| 19 |
STELLENBOSCH UNIVERSITY
Organization address
address: RYNEVELD STREET WILCOCKS BUILDING 2035 contact info |
ZA (STELLENBOSCH) | participant | 11˙640.00 |
| 20 |
LINCOLN UNIVERSITY
Organization address
address: Corner of Springs and Ellesmere Junction Road contact info |
NZ (Canterbury) | participant | 0.00 |
| 21 |
MINISTERE DE L AGRICULTURE DE L AGROALIMENTAIRE ET DE LA FORET
Organization address
address: Rue de Varenne 78 contact info |
FR (paris) | participant | 0.00 |
Mappa
Word cloud
Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.
Obiettivo del progetto (Objective)
'Development of accurate identification tools for plant pathogens and pests is vital to support European Plant Health Policies. For this project Council Directive 2000/29/EC is important, listing some 275 organisms for which protective measures against introduction into and their spread within the Community needs to be taken. Those threats are now greater than ever because of the increases in the volumes, commodity types and origins of trade, the introduction of new crops, the continued expansion of the EU and the impact of climate change. Currently identifying pathogens (in particular new emerging diseases) requires a staff with specialised skills in all disciplines (mycology, bacteriology, etc.); which is only possible within big centralised laboratory facilities. Taxonomy, phytopathology and other fields which are vital for sustaining sound public policy on phytosanitary issues are threatened with extinction. Modern molecular identification/detection techniques may tackle the decline in skills since they often require much less specialist skills to perform, are more amenable for routine purposes and can be used for a whole range of different target organisms. Recently DNA barcoding has arisen as a robust and standardised approach to species identification. QBOL wants now to make DNA barcoding available for plant health diagnostics and to focus on strengthening the link between traditional and molecular taxonomy as a sustainable diagnostic resource. Within QBOL collections harbouring plantpathogenic Q-organisms will be made available. Informative genes from selected species on the EU Directive and EPPO lists will be DNA barcoded from vouchered specimens. The sequences, together with taxonomic features, will be included in a new internet-based database system. A validation procedure on developed protocols and the database will be undertaken across worldwide partners to ensure robustness of procedures for use in a distributed network of laboratories across Europe'
Introduzione (Teaser)
EU researchers have created a DNA barcoding database of plant pathogens that is available to the public. It will help prevent economic losses from plant diseases.
Descrizione progetto (Article)
In the EU alone there are nearly 300 quarantine organisms for which protective measures need to be taken. To accurately identify plant pathogens and pests, specialised diagnostic tools are required.
Recently, DNA barcoding has emerged as an efficient way of marking organisms. The method uses a short piece of DNA, specific to a particular species, as a molecular DNA barcode.
The aim of the EU-funded http://www.qbol.org (QBOL) project was to generate such DNA-specific barcodes for quarantine organisms as a means of maintaining plant health. To this end, the consortium obtained gene sequences of species on the lists of the relevant EU directive and the European and Mediterranean Plant Protection Organization (EPPO).
Researchers created lists of quarantine organisms as well as other species that may pose a future threat to European plants. Based on these lists, they sourced genetic information for relevant fungi, insects, bacteria, viruses, nematodes and phytoplasmas (a type of bacterial parasite).
For each group, QBOL tested several DNA barcoding regions and selected the most reliable. Scientists also tested a number of different methods to extract DNA and chose the best one.
This information, along with the chosen DNA barcodes and supporting taxonomic information, was incorporated into a publically accessible http://www.q-bank.eu (database). Furthermore, diagnostic and bioinformatics tools were developed to enable the correct identification of quarantine organisms based on DNA barcode sequences.
Once established, the database was linked to the most-used online gene database, GenBank, as well as several other online resources. The functioning database was then refined based on general use and user feedback.