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QBOL

Development of a new diagnostic tool using DNA barcoding to identify quarantine organisms in support of plant health

Coordinatore	STICHTING DIENST LANDBOUWKUNDIG ONDERZOEK Organization address address: Costerweg 50 city: WAGENINGEN postcode: 6701BH contact info Titolo: Dr. Nome: Peter Cognome: Bonants Email: send email Telefono: +31 317 480648
Nazionalità Coordinatore	Netherlands [NL]
Totale costo	4˙142˙713 €
EC contributo	2˙991˙433 €
Programma	FP7-KBBE Specific Programme "Cooperation": Food, Agriculture and Biotechnology
Code Call	FP7-KBBE-2008-2B
Funding Scheme	CP-FP
Anno di inizio	2009
Periodo (anno-mese-giorno)	2009-03-21 - 2012-09-20

Partecipanti

#	participant	country	role	EC contrib. [€]
1	STICHTING DIENST LANDBOUWKUNDIG ONDERZOEK Organization address address: Costerweg 50 city: WAGENINGEN postcode: 6701BH contact info Titolo: Dr. Nome: Peter Cognome: Bonants Email: send email Telefono: +31 317 480648	NL (WAGENINGEN)	coordinator	564˙916.00
2	THE SECRETARY OF STATE FOR ENVIRONMENT, FOOD AND RURAL AFFAIRS Organization address address: Area 1A, Nobel House, Smith Square 17 city: LONDON postcode: SW1P 3JR contact info Titolo: Dr. Nome: Christina Cognome: Steveni Email: send email Telefono: -1904462182 Fax: -1904462207	UK (LONDON)	participant	390˙000.00
3	EIDGENOESSISCHES DEPARTEMENT FUER WIRTSCHAFT, BILDUNG UND FORSCHUNG Organization address address: Bundeshaus Ost city: BERN postcode: 3003 contact info Titolo: Mr. Nome: Roger Cognome: Fehr Email: send email Telefono: +41 44 783 62 05 Fax: +41 44 783 62 11	CH (BERN)	participant	345˙664.00
4	INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE Organization address address: Rue De L'Universite 147 city: PARIS CEDEX 07 postcode: 75338 contact info Titolo: Mr. Nome: Dominique Cognome: Ottomani Email: send email Telefono: +33 499 612 440 Fax: +33 499 612 882	FR (PARIS CEDEX 07)	participant	340˙060.00
5	KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPEN - KNAW Organization address address: KLOVENIERSBURGWAL 29 HET TRIPPENHUIS city: AMSTERDAM postcode: 1011 JV contact info Titolo: Mr. Nome: Don Cognome: Van Velzen Email: send email	NL (AMSTERDAM)	participant	305˙000.00
6	UNIVERSITEIT GENT Organization address address: SINT PIETERSNIEUWSTRAAT 25 city: GENT postcode: 9000 contact info Titolo: Ms. Nome: Nathalie Cognome: Vandepitte Email: send email Telefono: 32 (0)9 264 3029 Fax: -32 (0)9 264 3583	BE (GENT)	participant	210˙800.00
7	Vlaams Gewest Organization address address: KONING ALBERT II LAAN 20 city: BRUSSEL postcode: 1000 contact info Titolo: Prof. Nome: Erik Cognome: Van Bockstaele Email: send email Telefono: -2722479 Fax: -2722478	BE (BRUSSEL)	participant	210˙000.00
8	AARHUS UNIVERSITET Organization address address: Nordre Ringgade 1 city: AARHUS C postcode: 8000 contact info Titolo: Mr. Nome: Steen Lykke Cognome: Nielsen Email: send email Telefono: +45 89993500 Fax: +45 89993501	DK (AARHUS C)	participant	170˙000.00
9	ALMA MATER STUDIORUM-UNIVERSITA DI BOLOGNA Organization address address: Via Zamboni 33 city: BOLOGNA postcode: 40126 contact info Titolo: Dr. Nome: Torresan Cognome: Diego Email: send email Telefono: +39 051 2099373 Fax: +39 051 2098115	IT (BOLOGNA)	participant	169˙960.00
10	CENTRO INTERNACIONAL DE LA PAPA Organization address address: AVENIDA LA MOLINA 1895 city: Lima postcode: 12 contact info Titolo: Mr. Nome: Carlos Cognome: Alonso Email: send email Telefono: +51 1 317 3003 Fax: +51 1 317 5326	PE (Lima)	participant	110˙753.00
11	NEDERLANDSE VOEDSEL EN WARENAUTORITEIT Organization address address: CATHARIJNESINGEL city: UTRECHT postcode: 3540AA contact info Titolo: Ms. Nome: Marianne Cognome: Wiers Email: send email Telefono: 31 317 496654 Fax: 31 317 421701	NL (UTRECHT)	participant	65˙075.00
12	AGENCE NATIONALE DE SECURITE SANITAIRE DE L'ALIMENTATION, DE L'ENVIRONNEMENT ET DU TRAVAIL Organization address address: 14 RUE PIERRE ET MARIE CURIE city: Maisons Alfort postcode: 94701 contact info Titolo: Mrs. Nome: Nathalie Cognome: Franquet Email: send email Telefono: +33 2 41 20 74 22	FR (Maisons Alfort)	participant	15˙000.00
13	NACIONALNI INSTITUT ZA BIOLOGIJO Organization address address: VECNA POT 111 city: LJUBLJANA postcode: 1000 contact info Titolo: Dr. Nome: Maja Cognome: Ravnikar Email: send email Telefono: +386 1 42 333 88 Fax: +386 1 2573 847	SI (LJUBLJANA)	participant	14˙934.00
14	Chinese Academy of Inspection and Quarantine Organization address address: Chaoyang District 241 city: Beijing postcode: 1000123 contact info Titolo: Dr. Nome: Weimin Cognome: Li Email: send email Telefono: -85745816 Fax: -85755892	CN (Beijing)	participant	14˙400.00
15	INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA Organization address address: Carretera de la Coruna Km7.5 city: MADRID postcode: 28040 contact info Titolo: Dr. Nome: Jaime Cognome: Cubero Email: send email Telefono: 34913474162 Fax: -34913572293	ES (MADRID)	participant	14˙400.00
16	UNIVERSITA DEGLI STUDI DI MODENA E REGGIO EMILIA Organization address address: VIA UNIVERSITA 4 city: MODENA postcode: 41100 contact info Titolo: Prof. Nome: Emilio Cognome: Stefani Email: send email Telefono: -522545 Fax: -522614	IT (MODENA)	participant	13˙800.00
17	EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA Organization address address: Parque Estacao Biologica - PqEB s/n city: BRASILIA postcode: 70770901 contact info Titolo: Dr. Nome: Juliana Cognome: Freitas-Astua Email: send email Telefono: -11641 Fax: -4909	BR (BRASILIA)	participant	12˙825.00
18	Nome Ente NON disponibile Organization address address: State phytosanitary administration 23 city: Olomouc postcode: CZ77900 contact info Titolo: Dr. Nome: Katerina Cognome: Tomankova Email: send email Telefono: +420 585570155 Fax: +420 585227790	CZ (Olomouc)	participant	12˙206.00
19	STELLENBOSCH UNIVERSITY Organization address address: RYNEVELD STREET WILCOCKS BUILDING 2035 city: STELLENBOSCH postcode: 7601 contact info Titolo: Dr. Nome: Lizel Cognome: Mostert Email: send email Telefono: -8083391 Fax: -8084950	ZA (STELLENBOSCH)	participant	11˙640.00
20	LINCOLN UNIVERSITY Organization address address: Corner of Springs and Ellesmere Junction Road city: Canterbury postcode: 8150 contact info Titolo: Ms. Nome: Katrina Cognome: Wilke Email: send email Telefono: 0064 3 325 2811 ext 8974 Fax: 0064 3 325 3630	NZ (Canterbury)	participant	0.00
21	MINISTERE DE L AGRICULTURE DE L AGROALIMENTAIRE ET DE LA FORET Organization address address: Rue de Varenne 78 city: paris postcode: 75700 contact info Titolo: Mr. Nome: David Cognome: Caffier Email: send email Telefono: -241723207 Fax: -241482318	FR (paris)	participant	0.00

Mappa

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taken plant eppo barcoding barcodes lists qbol skills diseases created public recently diagnostic species molecular specialised quarantine taxonomic introduction selected tested barcode pathogens protective health q directive sequences tools gene online http database vital identification organisms pests dna taxonomy

Obiettivo del progetto (Objective)

'Development of accurate identification tools for plant pathogens and pests is vital to support European Plant Health Policies. For this project Council Directive 2000/29/EC is important, listing some 275 organisms for which protective measures against introduction into and their spread within the Community needs to be taken. Those threats are now greater than ever because of the increases in the volumes, commodity types and origins of trade, the introduction of new crops, the continued expansion of the EU and the impact of climate change. Currently identifying pathogens (in particular new emerging diseases) requires a staff with specialised skills in all disciplines (mycology, bacteriology, etc.); which is only possible within big centralised laboratory facilities. Taxonomy, phytopathology and other fields which are vital for sustaining sound public policy on phytosanitary issues are threatened with extinction. Modern molecular identification/detection techniques may tackle the decline in skills since they often require much less specialist skills to perform, are more amenable for routine purposes and can be used for a whole range of different target organisms. Recently DNA barcoding has arisen as a robust and standardised approach to species identification. QBOL wants now to make DNA barcoding available for plant health diagnostics and to focus on strengthening the link between traditional and molecular taxonomy as a sustainable diagnostic resource. Within QBOL collections harbouring plantpathogenic Q-organisms will be made available. Informative genes from selected species on the EU Directive and EPPO lists will be DNA barcoded from vouchered specimens. The sequences, together with taxonomic features, will be included in a new internet-based database system. A validation procedure on developed protocols and the database will be undertaken across worldwide partners to ensure robustness of procedures for use in a distributed network of laboratories across Europe'

Introduzione (Teaser)

EU researchers have created a DNA barcoding database of plant pathogens that is available to the public. It will help prevent economic losses from plant diseases.

Descrizione progetto (Article)

In the EU alone there are nearly 300 quarantine organisms for which protective measures need to be taken. To accurately identify plant pathogens and pests, specialised diagnostic tools are required.

Recently, DNA barcoding has emerged as an efficient way of marking organisms. The method uses a short piece of DNA, specific to a particular species, as a molecular DNA barcode.

The aim of the EU-funded http://www.qbol.org (QBOL) project was to generate such DNA-specific barcodes for quarantine organisms as a means of maintaining plant health. To this end, the consortium obtained gene sequences of species on the lists of the relevant EU directive and the European and Mediterranean Plant Protection Organization (EPPO).

Researchers created lists of quarantine organisms as well as other species that may pose a future threat to European plants. Based on these lists, they sourced genetic information for relevant fungi, insects, bacteria, viruses, nematodes and phytoplasmas (a type of bacterial parasite).

For each group, QBOL tested several DNA barcoding regions and selected the most reliable. Scientists also tested a number of different methods to extract DNA and chose the best one.

This information, along with the chosen DNA barcodes and supporting taxonomic information, was incorporated into a publically accessible http://www.q-bank.eu (database). Furthermore, diagnostic and bioinformatics tools were developed to enable the correct identification of quarantine organisms based on DNA barcode sequences.

Once established, the database was linked to the most-used online gene database, GenBank, as well as several other online resources. The functioning database was then refined based on general use and user feedback.