SYSTEMS MICROSCOPY
Systems microscopy – a key enabling methodology for next-generation systems biology
| Coordinatore | KAROLINSKA INSTITUTET
Organization address
address: Nobels Vag 5 contact info |
| Nazionalità Coordinatore | Sweden [SE] |
| Sito del progetto | http://www.systemsmicroscopy.eu |
| Totale costo | 15˙964˙673 € |
| EC contributo | 11˙986˙690 € |
| Programma | FP7-HEALTH
Specific Programme "Cooperation": Health |
| Code Call | FP7-HEALTH-2010-two-stage |
| Funding Scheme | NoE |
| Anno di inizio | 2011 |
| Periodo (anno-mese-giorno) | 2011-01-01 - 2015-12-31 |
Partecipanti
| # | ||||
|---|---|---|---|---|
| 1 |
KAROLINSKA INSTITUTET
Organization address
address: Nobels Vag 5 contact info |
SE (STOCKHOLM) | coordinator | 3˙061˙962.98 |
| 2 |
EUROPEAN MOLECULAR BIOLOGY LABORATORY
Organization address
address: Meyerhofstrasse 1 contact info |
DE (HEIDELBERG) | participant | 3˙398˙234.81 |
| 3 |
WEIZMANN INSTITUTE OF SCIENCE
Organization address
address: HERZL STREET 234 contact info |
IL (REHOVOT) | participant | 1˙640˙693.41 |
| 4 |
INSTITUT FUER MOLEKULARE BIOTECHNOLOGIE GMBH
Organization address
address: Dr Bohrgasse 3 contact info |
AT (VIENNA) | participant | 838˙673.60 |
| 5 |
UNIVERSITEIT LEIDEN
Organization address
address: RAPENBURG 70 contact info |
NL (LEIDEN) | participant | 780˙800.23 |
| 6 |
HELSINGIN YLIOPISTO
Organization address
address: YLIOPISTONKATU 4 contact info |
FI (HELSINGIN YLIOPISTO) | participant | 775˙421.01 |
| 7 |
UNIVERSIDAD DE MALAGA
Organization address
address: CALLE EL EJIDO S/N contact info |
ES (MALAGA) | participant | 671˙407.08 |
| 8 |
Idea Bio-Medical Ltd.
Organization address
address: Prof. Bergman St. 2 contact info |
IL (Rehovot) | participant | 340˙443.22 |
| 9 |
ASSOCIATION POUR LA RECHERCHE ET LE DEVELOPPEMENT DES METHODES ET PROCESSUS INDUSTRIELS - ARMINES
Organization address
address: Boulevard Saint-Michel 60 contact info |
FR (PARIS) | participant | 200˙000.00 |
| 10 |
FUNDACIO INSTITUT DE CIENCIES FOTONIQUES
Organization address
address: AVINGUDA CARL FRIEDRICH GAUSS 3 contact info |
ES (Castelldefels) | participant | 200˙000.00 |
| 11 |
EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZURICH
Organization address
address: Raemistrasse 101 contact info |
CH (ZUERICH) | participant | 79˙053.66 |
Mappa
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Obiettivo del progetto (Objective)
Biological processes occur in space and time, but current experimental methods for systems biology are limited in their ability to resolve this spatiotemporal complexity of life. In addition, traditional “omics” methods often suffer from limited sensitivity and need to average over populations of cells at the expense of cell to cell variation. Next-generation systems biology therefore requires methods that can capture data and build models in four dimensions, three-dimensional space and time, and needs to address dynamic events in single living cells. In fact, recent advances in automated fluorescence microscopy, cell microarray platforms, highly specific probes, quantitative image analysis and data mining provide a powerful emerging technology platform to enable systems biology of the living cell. These imaging technologies, here referred to as “Systems microscopy”, will be a cornerstone for next-generation systems biology to elucidate and understand complex and dynamic molecular, sub-cellular and cellular networks. As a paradigm to enable systems biology at the cellular scale of biological organization, this NoE will have as its core biological theme two basic but complex cellular processes that are highly relevant to human cancer: cell division and cell migration. Methods, strategies and tools established here will be applicable to many disease-associated processes and will be instrumental for obtaining a systems level understanding of the molecular mechanisms underlying human diseases as manifested at the living cell level. Through close multidisciplinary collaborations in our programme of joint activities this NoE will develop a powerful enabling platform for next-generation systems biology and will apply these tools to understand cellular systems underlying human cancer. This provides a unique opportunity for Europe to acquire a global lead in systems microscopy.
Introduzione (Teaser)
A multidisciplinary consortium of 15 groups is working to develop methodology utilizing high resololution microscopy data for systems biology. Systems microscopy emerges as a powerful tool for unveiling cell migration and division.
Descrizione progetto (Article)
Manner in which complex biological processes are tightly regulated in space and time dictates normal or abnormal physiology. Scientists need to record and integrate multiparametric data of spatial and temporal dimensions to differentiate between normal and diseased processes.
With this in mind, the EU-funded 'Systems microscopy - a key enabling methodology for next-generation systems biology' (SYSTEMS MICROSCOPY) project is developing a technological platform for studying single cells in three-dimensional (3D) space and time. It is based on advanced light microscopy to produce high-content data from images.
One of the network's objectives is to develop a novel pan-European microscopy infrastructure for systems biology. This includes new imaging platforms and software, as well as methods for statistics, bioinformatics and modelling of systems biology. Cellular dynamics are studied in a multidimensional manner to maximise the amount of information that can be extracted from the imaging of live cells.
The network is particularly interested in delineating the processes of cell division and cell migration implicated in cancer biology. For this purpose, they are using time-lapse imaging to visualise the effect of down-regulating the expression of several hundred mitotic genes at the single-cell level.
Furthermore, scientists have developed a model to simulate the process of mitosis through the clustering and classification of cell division movies. Testing of the translational applicability of the SYSTEMS MICROSCOPY approach has so far identified the deregulation of a set of mitosis-related genes in basal breast cancer samples.
Project methods, strategies and tools have the potential to be applied to many disease-associated processes. The unique features of systems microscopy promises its fast integration in the research field of systems biology as well as identifying the mechanisms of drug action.