CHROMATINDAMAGE

Regulation of chromatin compaction in response to DNA damage in mammalian cells

Coordinatore	INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM)    more  Organization address address: 101 Rue de Tolbiac city: PARIS postcode: 75654 contact info Titolo: Ms. Nome: Laurie Cognome: Louis-Joseph Email: send email Telefono: +33 1 45 17 29 32 Fax: +33 1 45 17 29 11
Nazionalità Coordinatore	France [FR]
Totale costo	185˙248 €
EC contributo	185˙248 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2010-IEF
Funding Scheme	MC-IEF
Anno di inizio	2011
Periodo (anno-mese-giorno)	2011-03-01   -   2013-02-28

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM)  Organization address address: 101 Rue de Tolbiac city: PARIS postcode: 75654 contact info Titolo: Ms. Nome: Laurie Cognome: Louis-Joseph Email: send email Telefono: +33 1 45 17 29 32 Fax: +33 1 45 17 29 11	FR (PARIS)	coordinator	185˙248.00
2	INSTITUT CURIE  Organization address address: 26, rue d'Ulm city: PARIS postcode: 75248 contact info Titolo: Ms. Nome: Corinne Cognome: Cumin Email: send email Telefono: +33 1 56 24 66 20 Fax: +33 156 24 66 27	FR (PARIS)	participant	0.00

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 Obiettivo del progetto (Objective)

'Protection of genome integrity is crucial for maintaining cell viability and preventing cancer. However, how the dynamic organisation of DNA into chromatin in the cell nucleus is orchestrated in order to allow and promote detection and repair of DNA lesions remains poorly understood. A controlled accessibility of damaged chromatin is likely critical for an efficient cellular response to DNA damage, but underlying molecular mechanisms enabling this regulation have to be deciphered. In this proposal, we describe how we will combine established techniques and a novel approach based on FLIM-FRET microscopy to quantitatively visualise chromatin compaction/decompaction in human cells and to analyse how this is regulated during the response to genotoxic stress. First, we will examine the spatio-temporal dynamics of chromatin re-organisation upon global or localised DNA damage. Then, we plan to characterise the factors involved in each step, with a particular focus on chromatin remodeling factors, in order to determine their exact functional importance for DNA damage signaling and repair. This study should provide mechanistic insight into how genome integrity is preserved in a chromatin context.'