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3D-Plant2Cells TERMINATED

Exploring the Impact of Pesticide on the 3D Metabolome and the Microbiota from the Whole Plant to the Cell Scale

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

3D-Plant2Cells project word cloud

Explore the words cloud of the 3D-Plant2Cells project. It provides you a very rough idea of what is the project "3D-Plant2Cells" about.

commensal plants nanodesi collected plant groups 3d innovative laser pool desorption resolved serial micro imaging microbiota sections explore communities human ms received interdisciplinary plant2cells profiled uploaded cultivation cell electrospray ionization scales fruits dimension topological maldi samples chamber bioinformatic assisted map cultivated contribution amplicon proposes representing analyzing safety hypothesis fruit sequence treatment entire treatments grown food consumption inputs model cellular pesticide tandem conventional hr mass explored metabolite surface organism visualize specimens nanodesorption microbiome matrix content chemistry mode agricultural metabolome spectrometry resolution interdisplinary microbes crops managements goals

Project "3D-Plant2Cells" data sheet

The following table provides information about the project.

Coordinator	CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS Organization address address: RUE MICHEL ANGE 3 city: PARIS postcode: 75794 website: www.cnrs.fr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	France [FR]
Project website	https://github.com/lfnothias/3d-plant2cells
Total cost	246˙668 €
EC max contribution	246˙668 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2015
Funding Scheme	MSCA-IF-GF
Starting year	2017
Duration (year-month-day)	from 2017-01-01 to 2019-12-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS Organization address address: RUE MICHEL ANGE 3 city: PARIS postcode: 75794 website: www.cnrs.fr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	FR (PARIS)	coordinator	246˙668.00
2	THE REGENTS OF THE UNIVERSITY OF CALIFORNIA THE REGENTS OF THE UNIVERSITY OF CALIFORNIA Organization address address: FRANKLIN STREET 1111, 12 FLOOR city: OAKLAND CA postcode: 94607 website: http://www.universityofcalifornia.edu/ contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	US (OAKLAND CA)	partner	0.00

Map

Project objective

The project 3D-Plant2Cells proposes to explore the impact of the agricultural mode on crops grown for human consumption. We are going to develop innovative interdisciplinary approaches to study the metabolome and the microbiota of plants in three-dimension (3D) at three different scales: the whole plant, the fruit, and the cellular scales. The objective of this study is to get a new insight on the metabolome and microbiota response to pesticide treatment. The hypothesis is that the chemistry and microbiome could change upon the use of conventional cultivation managements. To explore this hypothesis, two groups of plants will be cultivated in growth chamber and one will received pesticide treatments. Then, we will use an innovative interdisplinary approach to explore the entire pool of metabolite (metabolome) and the plant-associated commensal microbes (plant microbiota) on the whole plant surface (plant scale). Samples will be collected on the whole plant and their metabolite content will be profiled by high-resolution tandem-mass spectrometry (HR-MS/MS) and plant-associated micro-organism communities by amplicon sequence analysis. Results of these both inputs will be uploaded into a bioinformatic model representing a 3D topological map of the metabolome/microbiome over the entire organism surface. In a second part of the research project, we will study fruits of the cultivated specimens by 3D mass spectrometry imaging. The goals are to visualize, in a spatially-resolved way, the metabolome/microbiota response to pesticide treatments along with the pesticide distribution in 3D. The metabolome will be investigated at two scales: the fruit and the cell. The fruit scale will be explored by analyzing serial sections using matrix assisted laser desorption ionization (MALDI) or nanodesorption electrospray ionization (nanoDESI) .... The entire project will be an important contribution to assess the impact of pesticide on plant metabolome/microbiome and on food safety.

Publications

List of publications.
year	authors and title	journal	last update
2016	LF Nothias, I Protsiuc, T Alexandrov, R Knight, A Brunelle, D Touboul, PC Dorrestein 3D-Plant2cells project: Spatial metabolomic, spatial metagenomic, and 3D mass spectrometry imaging, to explore the impact of pesticides on plant metabolome and microbiota published pages: S1-S381, ISSN: 0032-0943, DOI: 10.1055/s-0036-1596119	Planta Medica 81/S 01	2020-04-08

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