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UTPE PEB SIGNED
Ultrahigh-throughput protein evolution for polyethylene biodegradation
Total Cost €
0EC-Contrib. €
0Partnership
0Views
0UTPE PEB project word cloud
Explore the words cloud of the UTPE PEB project. It provides you a very rough idea of what is the project "UTPE PEB" about.
undertaken
genetically
abundantly
mutagenesis
throughput
concentration
effort
evolution
sequence
sized
degradation
evolutionarily
scattering
rates
measuring
synergize
group
rate
waste
space
dr
associate
polyethylene
first
drop
protein
differential
assay
cambridge
slow
microfluidics
express
sequencing
generation
strains
directed
plastic
enzymes
enzyme
synthetic
assays
cytometry
sufficiently
pollution
degrading
biodegradation
practical
modern
convey
tech
cover
century
standard
picoliter
screens
molecular
ultrahigh
personal
persist
secondments
microbial
isolate
pe
droplet
overcome
inefficient
polymer
water
random
environmental
solution
genotypes
skills
techniques
bioinformatics
efficiency
phenotype
biotechnology
library
tractable
biochemistry
degraded
light
microbiology
desired
nature
chassis
facilities
ucl
sme
ways
optimise
droplets
hollfelder
university
ebi
extensive
oil
host
genotype
multidisciplinary
functionally
pressing
flow
Project "UTPE PEB" data sheet
The following table provides information about the project.
| Coordinator |
THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE
Organization address contact info |
| Coordinator Country | United Kingdom [UK] |
| Total cost | 224˙933 € |
| EC max contribution | 224˙933 € (100%) |
| Programme |
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility) |
| Code Call | H2020-MSCA-IF-2019 |
| Funding Scheme | MSCA-IF-EF-RI |
| Starting year | 2020 |
| Duration (year-month-day) | from 2020-08-01 to 2022-07-31 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE | UK (CAMBRIDGE) | coordinator | 224˙933.00 |
Map
Project objective
The build-up of plastic pollution is one of the most pressing environmental concerns. Polyethylene (PE), the most abundantly produced plastic polymer, can persist in nature for over a century. The microbial biodegradation of PE that has been observed is slow and inefficient. So far no effort has been undertaken to improve the efficiency of enzymes involved in the biodegradation of PE through directed protein evolution. Standard assays for measuring degradation rates are not sufficiently high-throughput to cover the sequence space required. I propose to use state-of-the-art protein evolution technology to overcome this problem in two ways. First, an ultrahigh-throughput microfluidics based approach, that can associate a given genotype with its phenotype in picoliter sized water-in-oil droplets, will be used to isolate the desired genotypes from a random mutagenesis library. Second, a novel assay for measuring polymer concentration within each droplet based on differential light scattering as the polymer is degraded will assay the PE degradation rate for a given enzyme. These techniques were developed in the research group of the proposed host, Dr. Hollfelder in the Department of Biochemistry at the University of Cambridge. Using these techniques, I will functionally express and evolutionarily optimise a range of PE degrading enzymes in genetically tractable host strains, creating a chassis to investigate the potential of microbial biodegradation as a solution to plastic waste. Secondments at the EBI, UCL and the SME Drop-Tech will convey practical skills in bioinformatics screens and droplet formation. The host group’s experience in enzyme biotechnology and directed protein evolution as well as its extensive modern facilities for microfluidics, next generation sequencing and flow cytometry will synergize with my personal research experience in synthetic, molecular and microbiology to find a multidisciplinary solution to the growing problem of plastic degradation.
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