PM-NANOMAPS

Dynamic spatial organization of plasma membrane proteins at the nanoscale

 Coordinatore JULIUS-MAXIMILIANS UNIVERSITAET WUERZBURG 

 Organization address address: SANDERRING 2
city: WUERZBURG
postcode: 97070

contact info
Titolo: Mr.
Nome: Christian
Cognome: Gloggengießer
Email: send email
Telefono: +49 9313182294
Fax: +49 9313187180

 Nazionalità Coordinatore Germany [DE]
 Totale costo 161˙968 €
 EC contributo 161˙968 €
 Programma FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call FP7-PEOPLE-2013-IEF
 Funding Scheme MC-IEF
 Anno di inizio 2014
 Periodo (anno-mese-giorno) 2014-09-01   -   2016-08-31

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    JULIUS-MAXIMILIANS UNIVERSITAET WUERZBURG

 Organization address address: SANDERRING 2
city: WUERZBURG
postcode: 97070

contact info
Titolo: Mr.
Nome: Christian
Cognome: Gloggengießer
Email: send email
Telefono: +49 9313182294
Fax: +49 9313187180

DE (WUERZBURG) coordinator 161˙968.80

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

trafficking    membrane    microscopy    compartments    skeleton    adhesion    rafts    proteins    function    fluorescence    induced    turnover    transduction    cell    protein    insights    spatial    pm    actin    nanoclusters    lipid    signal    dynamic    organization   

 Obiettivo del progetto (Objective)

'Besides its function as a passive cell wall plasma membrane (PM), involved in signal transduction and cell adhesion, is essential to enable the formation of tissues. Understanding PM function requires molecular insights into its dynamic spatial organization. Recent studies show that membrane proteins rearrange laterally forming nanoclusters of various size. On the other hand, a hierarchical model of two dimensional PM organization has been proposed including transient confinement in membrane-actin-skeleton induced compartments and lipid rafts. However, an overall picture of the dynamic spatial organization of the PM requires the inclusion of protein trafficking since membrane proteins undergo a constitutive turnover transported in vesicles as cargo from and to the cytosol.

The hypothesis of this project is that vesicle trafficking to and from the PM is responsible for the observation of nanoclusters in the PM. Due to the diffraction barrier it is impossible to image nanoclusters with conventional fluorescence microscopy. To avoid this limitation, I will use super-resolution fluorescence imaging methods such as direct Stochastic Optical Reconstruction Microscopy (dSTORM) and Photoactivated Localization Microscopy (PALM) combined with novel statistical cluster analysis to study quantitatively the dynamic reorganization of PM induced by protein trafficking, as well as the role played by actin-skeleton-induced compartments and lipid rafts during this process. I will apply this approach to study a wide family of membrane proteins involved in signal transduction and cell adhesion essential for embryogenesis, neurogenesis, and immune response. The outcome of the project is expected to provide fundamental new insights into PM architecture and organization including protein turnover. Since membrane proteins are one of the most attractive drug targets because they control the communication of cells with their environment, the results will be of high medical significance.'

Altri progetti dello stesso programma (FP7-PEOPLE)

HOLOLAND (2014)

"Charting the holographic quantum landscape, and consequences on charge transport at strong coupling"

Read More  

UMBEL (2014)

"E-taxonomy of Sino-Himalayan Umbelliferae (Apiaceae): diversity, phylogeny and species modelling through new web-based tools"

Read More  

CORCON (2014)

Correctness by Construction

Read More