PAX5TARGETS
CHARACTERIZATION OF THE B-CELL-SPECIFIC GENE REGULATORY NETWORK BY GENOME-WIDE IDENTIFICATION OF DIRECT PAX5 TARGET GENES
| Coordinatore | FORSCHUNGSINSTITUT FUER MOLEKULARE PATHOLOGIE Ges.m.b.H
Organization address
address: Dr. Bohr-Gasse 7 contact info |
| Nazionalità Coordinatore | Austria [AT] |
| Totale costo | 160˙749 € |
| EC contributo | 160˙749 € |
| Programma | FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) |
| Code Call | FP7-PEOPLE-2007-2-1-IEF |
| Funding Scheme | MC-IEF |
| Anno di inizio | 2008 |
| Periodo (anno-mese-giorno) | 2008-03-01 - 2010-02-28 |
Partecipanti
| # | ||||
|---|---|---|---|---|
| 1 |
FORSCHUNGSINSTITUT FUER MOLEKULARE PATHOLOGIE Ges.m.b.H
Organization address
address: Dr. Bohr-Gasse 7 contact info |
AT (VIENNA) | coordinator | 0.00 |
Mappa
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Obiettivo del progetto (Objective)
'A complex network of transcription factor interactions controls the process by which B lymphocytes develop from hematopoietic stem cells (HSCs). Pax5 has been identified as the critical transcription factor controlling B cell commitment throughout B lymphopoiesis. These analyses revealed that Pax5 commits early hematopoietic progenitors to the B cell pathway by repressing B-lineage-inappropriate genes and by simultaneously activating B-cell-specific genes. By comprehensive cDNA microarray analysis, 110 Pax5-repressed and 170 Pax5-activated genes have been identified, demonstrating that Pax5 controls a complex regulatory network involved in B cell signaling, adhesion, migration and immune function. The goal of the project proposed here will be to systematically analyze all direct Pax5 target genes in the entire genome during B cell development. Recently, a mouse strain carrying a biotin tag at the C-terminus of Pax5 has been generated. We will use this mouse strain for genome-wide identification of Pax5-binding sites by chromatin precipitation and Solexa 1G ultrahigh-throughput DNA sequencing. Islands of chromatin modifications have been shown to provide an efficient way of identifying active and repressed promoters and cis-regulatory elements. Here we propose to use chromatin immunoprecipitation (ChIP)-sequencing to map the entire mouse genome for Pax5-controlled promoters and cis-regulatory elements. Integration of this information with the identification of Pax5-binding sites will provide us with a genome-wide map of functional Pax5-binding sites, from which a list of all direct target genes of Pax5 during B cell development will be obtained. The project proposed here will constitute an essential piece of a larger group effort, which aims at elucidating the gene regulatory network interactions controlling B cell development.'
Introduzione (Teaser)
Pax proteins are important regulators in early development as well as neural development and spermatogenesis. Changes in their gene expression are believed to contribute to the growth of malignant tumours.